Laboratory to study the mechanisms of carcinogenesis and anticarcinogenesis

Research areas

Identification of the danger of environmental exposure and evaluation of the risk of adverse outcomes, with particular attention to tumor pathology. The hazard assessment is performed according to the convention, using the Arpae-ER CTR (primary: Dr. Annamaria Colacci), which is housed in the Cancerology section of DIMES. The convention, focused on the research project 'Environmental risks and human health', has created strategies and experimental models using an integrated approach consisting of in vitro and toxico-genomic testing. This approach has made it possible to combine human resources, instrumentation, and scientific objectives, allowing university personnel access to the CTR labs in Arpae-ER (Laboratorio Mater di tossicogenomica e Centro di Saggio “Vitrox” certificato in Buona Pratica di Laboratorio, laboratorio di riferimento commissione europea, rete EU-NETVAL).

The risk assessment is performed using BMDS software to estimate the quantitative carcinogenic risk for mutagenic carcinogens, by calculating the TDI for substances with threshold dose. The use of this approach 

has facilitated the elaboration of various models in the OECD working plan, the active participation of national and international working groups in the creation of guidelines, testing strategies, and the risk assessment of dangerous substances.

Focus of financed projects: a) Progetto PaMPER); b) Thyroid Project – TR Calux validation

Teaching activity

Annamaria Colacci, Adjunct professor in Environmental Hygiene,  LT-  Scienze Biologiche, Scuola di Scienze, Università di Bologna; and Adjunct professor of  Applied Hygiene, LM- Biologia della Salute, Scuola di Farmacia, Università di Bologna

Responsible for teaching and organizing the following courses:

  • Nutrition and health education (Master II Level)
  •  Management of environmental control (Master I Level)

Monica Vaccari,  Management of environmental control (Master I Level)

 

Available instrumentation

  • CO2 incubators
  • LAminar flow fume hood BL2
  • Inverted microscopes
  • Freezer at -80°C
  • Liquid nitrogen containers for the storage of cells
  • Tools for micro-injection and -manipulation; at the headquarters of Arpae-ER (via Rocchi 19), the university group  has been given the possibility of using them for in vitro toxico-genomics and experimentation in BPL

 

Services and consulting

Annamaria Colacci

  • OECD: Cell Transformation Expert Panel (CTA);  Endocrine Disruptor Testing and Assessment Advisory Group, (EDTA);  Extended Advisory Group on Molecular Screening and Toxico-genomics (EAGMST);  Validation Management Group for Non-Animal Testing (VMG); Integrated Approach for Testing and Assessment (IATA) on non-genotoxic carcinogenesis – Steering Committee
  • WHO – International  Chemical Safety Cards Program
  • Rete EIONET – European Environmental Agency
  • EU Commission – Expert Advisory Group on Endocrine Disruptors
  • EU Commission -  International Cooperation for Alternative Testing Methods (ICATM)
  • ANSES – Groupe d’expertise  collective d’urgence - Francia Nazionali
  • Task Force Ambiente e Salute – Ministero della Salute
  • Osservatorio PFAS – Ispra – Ministero dell’Ambiente
  • Ispra SNPA TIC VII Ricerca e Sviluppo – coordinatore GdL 07 – Esposizione
  • GdL Emergenze – Regione Emilia Romagna
  • GdL Profilo della salute – Regione Emilia Romagna
  • GdL CEM – Comune di Bologna

Monica Vaccari

  • OECD:  Cell Transformation Expert Panel (CTA)
  • Integrated Approach for Testing and Assessment (IATA) on non-genotoxic carcinogenesis
  • Expert Group on Transcriptomics-Transcriptomic Reporting Framework Group

Principal Investigator

Team

Giovanni Ammirante

B cat. - general and technical services area

Abd Husson Ali Hade

B cat. - general and technical services area

How to reach us

Laboratory to study the mechanisms of carcinogenesis and anticarcinogenesis

viale Filopanti 22, Bologna